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KMID : 1134820100390081119
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 8 p.1119 ~ p.1125
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Resveratrol Induces Apoptosis in Primary Human Prostate Cancer Cells
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Kang Hye-In
Kim Jae-Yong
Cho Hyun-Dong
Park Kyung-Wuk
Kang Jum-Soon
Seo Kwon-Il
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Abstract
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To evaluate resveratrol as a prostate cancer preventive material, we investigated its anti-proliferative and apoptotic effects in RC-58T/h/SA#4 primary human prostate cancer cells. Resveratrol significantly decreased the number of viable RC-58T/h/SA#4 cells in a dose- and time-dependent manner. Resveratrol showed cytotoxicity against RC-58T/h/SA#4, LNCaP, PC-3 human prostate cancer cells with IC50 values of 245, 320 and 340 μM, respectively. However the cytotoxic potential of resveratrol against normal RWPE-1 cells was lower (IC50=982 μM). Resveratrol induced cell death as evidenced by the increased formation of apoptotic bodies, nuclear condensation, sub-G1 phase, and DNA fragmentation. Resveratrol activated initiator caspases 8, and 9 as well as effector caspase 3 in a dose-dependent manner. Furthermore, the general caspase inhibitor z-VAD-fmk significantly inhibited resveratrol-induced apoptosis compared to cells without treatment. These results clearly indicate that resveratrol-induced apoptosis was dependent on caspase activation. Further, resveratrol modulated the down regulation of Bcl-2 (anti-apoptotic), and Bid. However, the level of Bax (pro-apoptotic) remained unchanged. These results suggest that resveratrol induced apoptosis in RC-58T/h/SA#4 cells via a mitochondrial-mediated caspase-dependent pathway, suggesting therapeutic potential against prostate cancer.
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KEYWORD
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resveratrol, prostate cancer cell, RC-58T/h/SA#4, apoptosis
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